Born in 1969 and raised in Essen, Germany, Tilo Werner studied acting at the Ernst Busch Academy of Dramatic Arts in Berlin. After his third year of studies, he was engaged by the Maxim Gorki Theater in Berlin. Following Thomas Ostermeier and Jens Hillje's appointment to the renowned Baracke stage, he joined the Deutsches Theater and subsequently the Schaubühne am Lehniner Platz.
Inspired by an encounter with the Hungarian director Árpád Schilling, Werner moved to Budapest, learned Hungarian, and joined the Krétakör Theatre. He later became an ensemble member at Vienna's Burgtheater while remaining closely connected to his adopted home of Budapest, appearing in numerous Hungarian short and feature films.
Since the 2009/10 season, Tilo Werner has been a permanent ensemble member of the Thalia Theater in Hamburg. Throughout his career, he has collaborated with acclaimed directors including Luc Perceval, Dimiter Gotscheff, Kornél Mundruczó, and Kirill Serebrennikov. He also works regularly as a voice actor for radio dramas and documentary features.